뼈를 형성을 조절하는 조골세포, 상아질의 형성을 조절하는 상아모세포에서 광화 (mineralization)와 연관된 유전자의 발현을 조절하는 전사인자와 그들의 조절 기전을 연구하여, 뼈형성이 필요한 골다공증, 치주질환 등의 새로운 치료방법을 개발하고자 함
뼈를 형성을 조절하는 조골세포, 상아질의 형성을 조절하는 상아모세포에서 광화 (mineralization)와 연관된 유전자의 발현을 조절하는 전사인자와 그들의 조절 기전을 연구하여, 뼈형성이 필요한 골다공증, 치주질환 등의 새로운 치료방법을 개발하고자 함
1. 유전자적중 마우스를 이용한 유전자 기능연구
2. Runx2, Dlx5, Osx, Msx2 등 골형성 관련 전사인자 활성연구
3. 골형성, 골광화 촉진물질 screening system
4. PIN1에 의한 RUNX2 단백질의 Posttranslational modification (PTM): 활성 강화, 안정성 증가
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Core binding factor β of osteoblasts maintains cortical bone mass via stabilization of Runx2 in mice. J Bone Miner Res. 2015 Apr; 30(4):715-22. 10: Yoon WJ, Islam R, Cho YD, Ryu KM, Shin HR, Woo KM, Baek JH, Ryoo HM. Pin1 plays a critical role as a molecular switch in canonical BMP signaling. J Cell Physiol. 2015 Mar; 230(3):640-7. 11: Woo KM, Jung HM, Oh JH, Rahman SU, Kim SM, Baek JH, Ryoo HM. Synergistic effects of dimethyloxalylglycine and butyrate incorporated into α-calcium sulfate on bone regeneration. Biomaterials. 2015 Jan; 39:1-14. 12: Islam R, Bae HS, Yoon WJ, Woo KM, Baek JH, Kim HH, Uchida T, Ryoo HM. Pin1 regulates osteoclast fusion through suppression of the master regulator of cell fusion DC-STAMP. J Cell Physiol. 2014 Dec; 229(12):2166-74. 13: Yoon H, Kim HL, Chun YS, Shin DH, Lee KH, Shin CS, Lee DY, Kim HH, Lee ZH, Ryoo HM, Lee MN, Oh GT, Park JW. NAA10 controls osteoblast differentiation and bone formation as a feedback regulator of Runx2. Nat Commun. 2014 Nov 7;5:5176. 14: Lee DS, Choung HW, Kim HJ, Gronostajski RM, Yang YI, Ryoo HM, Lee ZH, Kim HH, Cho ES, Park JC. NFI-C regulates osteoblast differentiation via control of osterix expression. Stem Cells. 2014 Sep;32(9):2467-79. 15: Cho YD, Yoon WJ, Kim WJ, Woo KM, Baek JH, Lee G, Ku Y, van Wijnen AJ, Ryoo HM. Epigenetic modifications and canonical wingless/int-1 class (WNT) signaling enable trans-differentiation of nonosteogenic cells into osteoblasts. J Biol Chem. 2014 Jul 18;289(29):20120-8 16: Islam R, Yoon WJ, Woo KM, Baek JH, Ryoo HM. Pin1-mediated prolyl isomerization of Runx1 affects PU.1 expression in pre-monocytes. J Cell Physiol. 2014 Apr;229(4):443-52. 17: Yoon WJ, Cho YD, Kim WJ, Bae HS, Islam R, Woo KM, Baek JH, Bae SC, Ryoo HM. Prolyl isomerase Pin1-mediated conformational change and subnuclear focal accumulation of Runx2 are crucial for fibroblast growth factor 2 (FGF2)-induced osteoblast differentiation. 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J Dent Res. 2010 Nov;89(11):1203-7. 26: Song GA, Kim HJ, Woo KM, Baek JH, Kim GS, Choi JY, Ryoo HM. Molecular consequences of the ACVR1(R206H) mutation of fibrodysplasia ossificans progressiva. J Biol Chem. 2010 Jul; 285(29):22542-53. 27: Park OJ, Kim HJ, Woo KM, Baek JH, Ryoo HM. FGF2-activated ERK mitogen-activated protein kinase enhances Runx2 acetylation and stabilization. J Biol Chem. 2010 Feb 5; 285(6):3568-74. 28: Cho YD, Yoon WJ, Woo KM, Baek JH, Lee G, Cho JY, Ryoo HM. Molecular regulation of matrix extracellular phosphoglycoprotein expression by bone morphogenetic protein-2. J Biol Chem. 2009 Sep 11;284(37):25230-40. 29: Yoon WJ, Cho YD, Cho KH, Woo KM, Baek JH, Cho JY, Kim GS, Ryoo HM. The Boston-type craniosynostosis mutation MSX2 (P148H) results in enhanced susceptibility of MSX2 to ubiquitin-dependent degradation. J Biol Chem. 2008 Nov 21;283(47):32751-61. 30: Kim BG, Kim HJ, Park HJ, Kim YJ, Yoon WJ, Lee SJ, Ryoo HM, Cho JY. 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